fast activated cell-based elisa (facetm) kits  (Active Motif)

Journal: Frontiers in Oncology

Article Title: Inflammation and Invasion in Oral Squamous Cell Carcinoma Cells Exposed to Electronic Cigarette Vapor Extract

doi: 10.3389/fonc.2022.917862

Figure Lengend Snippet: Expression of NF- k B, pERK, and pJNK in Ca9-22 cells exposed to EVE. ELISA was performed to determine NF- k B released into the cell medium. ERK and JNK were determine by western blot. Released of NF- k B in the media was increased only with Apple EVE and nicotine EVE in treated Ca9-22 cells as compared to media only treated controls (A) . A representative western blot results for ERK and JNK us shown in (B) ERK protein was activated in cells treated with Apple EVE and Nicotine and significantly decreased in cells exposed to Red Hot EVE with nicotine (C) . JNK protein was activated by both flavors in Ca9-22 cells when nicotine was present (D) . Data are shown with * p ≤ 0.05.

Article Snippet: Released Nuclear factor kappa B (NF- k B) and Tumor necrosis factor alpha (TNF-α) levels were assessed in culture media (n=8) using colorimetric high-throughput fast activated cell-based ELISA assays available from Active Motif (Carlsbad, CA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Frontiers in Oncology

Article Title: Inflammation and Invasion in Oral Squamous Cell Carcinoma Cells Exposed to Electronic Cigarette Vapor Extract

doi: 10.3389/fonc.2022.917862

Figure Lengend Snippet: | Expression of NF- k B (A) , pERK (B) , and pJNK (C) in Cal27 cells exposed to EVE. ELISA was performed to determine NF- k B released into the cell medium. ERK and JNK were determine by western blot. Released NF- k B was decreased with both flavors and nicotine in these cells (A) as compared to media only treated controls cells. A representative western blot results for ERK and JNK is shown in (B) Activation of ERK and JUNK proteins protein was decreased after exposure to both flavoring with nicotine except for induction of pJNK by Red Hot (C, D) . Data are shown with * p ≤ 0.05.

Article Snippet: Released Nuclear factor kappa B (NF- k B) and Tumor necrosis factor alpha (TNF-α) levels were assessed in culture media (n=8) using colorimetric high-throughput fast activated cell-based ELISA assays available from Active Motif (Carlsbad, CA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Activation Assay

Journal: Frontiers in Oncology

Article Title: Inflammation and Invasion in Oral Squamous Cell Carcinoma Cells Exposed to Electronic Cigarette Vapor Extract

doi: 10.3389/fonc.2022.917862

Figure Lengend Snippet: Expression of TNF-α in OSCCs exposed to EVE. ELISA was performed to determine TNF-α released into the cell medium. Released TNF-α levels were increased in conditioned media from Ca9-22 (A) and Cal27 (B) exposed to both EVE flavors plus nicotine. Data are shown with * p ≤ 0.05.

Article Snippet: Released Nuclear factor kappa B (NF- k B) and Tumor necrosis factor alpha (TNF-α) levels were assessed in culture media (n=8) using colorimetric high-throughput fast activated cell-based ELISA assays available from Active Motif (Carlsbad, CA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: International Immunology

Article Title: Goblet cell-produced retinoic acid suppresses CD86 expression and IL-12 production in bone marrow-derived cells

doi: 10.1093/intimm/dxy045

Figure Lengend Snippet: Expression of inflammatory mediators by BMDCs. (A) Effects of goblet cell conditioned media (CjCM) or RA compared to control day 9 untreated group on relative fold expression of Th1 cytokines IL-12 and IFN-γ in cultured BMDCs with or without LPS treatment, measured by RT–PCR. Results expressed as mean ± SEM, n = 4, ***P < 0.005. (B) IL-12 intra-cellular staining of BMDCs detected by flow cytometry (mean ± SEM, n = 3). Untreated (UT; i.e. no LPS) versus LPS treated within group, *P < 0.05; †P < 0.05 comparison between day 9 UT versus CjCM UT. (C) Level of NF-kB p65 phosphorylation in DCs with/without CjCM or RA treatment. Day 9 BMDCs were seeded in 96-well plates treated without or with LPS for 45 min and phospho-p65 (ser536) and total p65 were measured by cell-based enzyme-linked immunosorbent assay. Results expressed as %phospho-p65/total p65 are mean ± SEM of three independent experiments. (D) Effects of CjCM or RA compared to control group on relative fold expression of chemokine receptor CCR7 and ICAM-1 in BMDCs (mean ± SEM, n = 4), ****P < 0.001. UT versus LPS within groups: *P < 0.05, **P < 0.01; between-group comparisons: †P < 0.05, ††P < 0.01, †††P < 0.005.

Article Snippet: NF-κB p65 activation was quantitatively measured by a Fast-activated cell-based ELISA (FACE™) NF-κB p65 Profiler Kit (Active Motif, Carlsbad, CA, USA) that specifically measures phosphorylated and total NF-κB p65.

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay